![]() ![]() In mice, homozygous Mitf mutations ( Mitf Mi/Mitf Mi) cause pigmentation defects and microphthalmia with thickening of the RPE that forms a multilayered structure resembling the early stage of neural retina ( 6, 8, 10, 17–19). In humans, heterozygous MITF mutations result in Waardenburg syndrome type IIA and Tietz syndrome that are characterized by hearing loss and pigmentation defects ( 15, 16). The MITF-TFE (MiT) subfamily of proteins, which includes MITF, TFE3, TFEB and TFEC, forms homodimers or heterodimers within the subfamily and binds to E-box sequences in vitro ( 6, 12). ![]() ![]() MITF is a member of the basic helix-loop-helix leucine zipper (bHLH-ZIP) family of transcription factors and is expressed in several cell lineages, including melanocytes, RPE, osteoclasts and mast cells ( 6, 10, 12–14). MITF and OTX2 co-localize in the nuclei of the RPE, interact with each other, and can cooperatively activate some genes, such as QNR71 and tyrosinase ( Tyr) ( 11). Two of the key transcription factors required for RPE specification and development are microphthalmia-associated transcription factor (MITF) and orthodenticle homeobox 2 (OTX2) ( 3–10). RPE shares its origin with the neural retina, as both tissues are derived from the neuroepithelium of the forebrain ( 3, 4). The retinal pigment epithelium (RPE), a monolayer of cuboidal cells with melanin pigment located between the photoreceptors and choroid of the eye, has many specialized functions that nourish and support retinal photoreceptors ( 1, 2). These results demonstrate for the first time CRX expression in the RPE, and suggest that OTX2 and CRX may act as positive modulators of the BEST1 promoter in the RPE. Surprisingly, we found that human and bovine RPE expressed not only OTX2 but also CRX, the CRX genomic region in bovine RPE was hypersensitive to DNase I, consistent with active transcription, and that both OTX2 and CRX bound to the BEST1 proximal promoter in vivo. Three OTX family proteins – OTX1, OTX2 and CRX – bound to both Sites 1 and 2 in vitro, and all of them increased BEST1 promoter activity. Since another non-canonical OTX site (Site 2) is located nearby, we tested the function of these sites using BEST1 promoter/luciferase constructs by in vivo electroporation and found that mutation of both sites reduces promoter activity. Here, we show that the −154 to −104 bp region is necessary for RPE expression in transgenic mice and contains a predicted OTX-binding site (Site 1). The human BEST1 upstream region from −154 to +38 bp is sufficient to direct expression in the RPE, and positive-regulatory elements exist between −154 and −104 bp. As a model for RPE gene regulation, we have been studying the mechanisms that control BEST1 expression, and recently demonstrated that members of the MITF–TFE family modulate BEST1 transcription. One of these, BEST1, encodes bestrophin-1, a protein that when mutated causes Best macular dystrophy. A number of genes preferentially expressed in the retinal pigment epithelium (RPE) are associated with retinal degenerative disease. ![]()
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![]() ![]() Type the ending balance from your statement in the Statement Balance field, near the bottom right of the window, at the top of the Statement column. It's a powerful app and I highly recommend it.Reconcile against a statement Click the Reconcile button at the bottom of the document window. And when you hit search it will bring up EVERYTHING related to what you put in there. You can search just the month, or one day, or the whole year. ![]() And you can choose the time frame that the search uses. If you search "food", you can see all the times you spent money on food. You can look up by category, keyword, bank account, and more. There is a wonderful search bar too, in case you needed to look something up. I love how I can see where every penny has gone or come in from. It is so detailed and precise and I have never been in so much control of my money. Especially since the app lays it out SO beautifully. By just taking an hour every couple of weeks and entering all my bank transactions, I can see what I'm doing with my money. This app has tremendously helped me with managing my money. I did upgrade to the paid version this year, but probably will not renew.ĭo you want to be on top of your finances? Track your everyday spending and what not? Then this app is for you. I’m bringing this up because I have contacted the designers twice but have never received a response. So I’m scrambling to find the disparity between the bank’s records (which show the payment) and my own (which show the next payment will be recorded in October even though the September payment was never recorded). But either way, the list of recurring payments rolls on to the next month. The app is set up to enter the payment into the register on the first of each month. For example, I have a regular monthly insurance payment. Others are rolled over without ever being entered. Some entries are duly entered into the register, then “rolled over” to the next month. But…the recurring entries function has simply stopped working reliably. Simple and flexible, and just what I wanted: a check register that makes it easy to keep my accounts balanced. If you have any question, please email to Acme shopper ⁃ Support both iCloud and Dropbox backup and restore. ⁃ Set Primary Account, primary category and home currency. ⁃ Export data inform of PDF, CSV and HTML. Monthly income & expense cash flow histogram. Total income & expense of all accounts and each account.ĭ). ⁃ Search transactions by name, category, account, amount, type and note text in a specific date range.Ī). ⁃ Supports adding recurring transactions. ⁃ Kinds of default categories and you can add your own. ⁃ Mark transaction as cleared or not directly in main screen page. ![]() ⁃ Supports splitting a transaction across multiple categories. ⁃ Supports adding expense, income and transfer transactions. ⁃ Show daily and monthly total income and expense by calendar. ⁃ Show transactions by daily and monthly. ⁃ Supports different currencies for each account. ⁃ Track total balance, cleared balance of total accounts and each account. You can set multiple currencies for this app, and it support iCloud auto sync, iCloud & Dropbox backup and restore, makes it easier for you and your other family number to manage accounts together. A simple and easy to use app to track transactions and balance of your accounts like checking saving, credit card and cash etc.Ĭheckbook Wiz is a convenient way to manage your personal daily finance, check or add transactions anywhere with your device, keep an eye on balance of your accounts, see clearly where you money comes and goes. ![]() ![]() ![]() Neko Atsume: Kitty Collector isn't really something you "play" so much as it is a simple pocketful of virtual pets you check in on once in a while, and if you want something absolutely adorable and low key to put a smile on your face, this is definitely it. All you need to do is check back in with the app periodically to refill their food dish, which is free, although different food types can be bought with fish, and snap new photos if you like, and after a cat has visited you enough to take a liking to you, they may leave you a special item trophy as an achievement. There are different types and colours of cats, some who parody pop culture, and your goal is simply to see and entice as many as you can. It's essentially Sea Monkeys, only, you know, better because it's cats, and they have cute little cat bums. ![]() If you're lucky, they'll leave golden fish, which can be spent on expanding the area or even better items, though if you're impatient you can buy more golden fish for optional in-app purchases. The cats, when they leave, leave you some fish in return for their fun and food, which can be then spent on buying more enhancements for the play area. You put food out in your backyard, along with some toys (and since they're cats, "toys" can also mean "boxes and bags"), and cats come to play with them. Become a master kitty collector and complete your collection. ![]() Each visiting cat is logged in your catbook. More than 20 varieties of cats white and black, tabby and calico might stop by. In Hit-Point's super simple sim, Neko Atsume: Kitty Collector, free for iOS and Android, is all about cats. Playing Neko Atsume VR is as easy as one-two-three Place toys and snacks in your yard and wait for cats to visit. Neko Atsume: Kitty Collector is now available in English! ![]() ![]() The song spawned a scathing rebuttal from Nyovest in the form of Dust 2 Dust. ![]() That’s my work, I have the files and everything, and it should be known that I have created it.”ĪKA released the song, whose lyrics are aimed at rival Cassper Nyovest, in September to much fanfare. I just want to be acknowledged as the producer of the beat. For the publishing market, we provide full-service publishing solutions, specializing in project management, editorial, design, production. “We don’t know what Gordon (Nkosi) is talking about, however that doesn’t mean we will let the matter slide. We will look into it perhaps have a listen to his original link to the beat, and the one used on the song,” Pheko Kgengoe from AKA’s public relations team said.Īlthough Nkosi admits to having blundered by not registering the beat, he insists all he wants is recognition for his work. A.K.A - Composure (traduo) (Letra e msica para ouvir) - One, two, one, two / Why you gassing dawg / Why you comin to my table without asking dawg / I. Composure is a comprehensive, creative solutions company. “I contacted John on the spot, and he said we would meet and work something out, which is something I was OK with, because no producer was credited on the song.”īut John reportedly went AWOL and switched off his phone soon after.ĪKA’s camp has since distanced themselves from the claims and say they don’t know and have never heard of Gordio G-Cover before. An affront which allegedly led to Nyovest attempting to confront AKA. “I immediately listened to the song and was stunned to hear a beat exactly the same as mine.” Last week, AKA had the upper hand when he performed his diss track, Composure, which is dedicated to Nyovest in front of him. It does not store any personal data.“I noticed that everyone was going on about Composure,” Nksoi said. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. One two one two Why you gassing dawg Why you coming to my table without asking dawg I know you hate me with a passion I ain't mad at y'all I know you fck with every classic in my catalog And it go worth your magazine team think it's lootlove Cashtime with the Sheen Team push dust I don't need a green screen when I pull stunts Cause when you. ![]() The cookie is used to store the user consent for the cookies in the category "Other. Lyrical master AKA who was recently nominated at the 2015 MTVEMA drops one on his rivals Cassper Nyovest & Anatii on. ![]() This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary". This cookie is set by GDPR Cookie Consent plugin. Letra de COMPOSURE de A.K.A.: Verse 1, Why you gassin dawg, Why you comin to my table without askin dawg, I know you hate me with a passion I aint. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". This cookie is set by GDPR Cookie Consent plugin. A.K.A - Composure (Letra e msica para ouvir) - One, two, one, two / Why you gassing dawg / Why you comin to my table without asking dawg / I know you hate. ![]() South African rapper AKA who just got nominated alongside Davido and Yemi Alade at the 2015 MTVEMA comes for. These cookies ensure basic functionalities and security features of the website, anonymously. AKA Composure (Cassper Nyovest & Anatii Diss). Necessary cookies are absolutely essential for the website to function properly. ![]() ![]() ![]() Over the years, Repairit has been a powerhouse for videographers, photographers, and even companies as it allows easy recovery of both video and photo files without tampering with their quality. This tool boasts a clean user interface that makes it very user-friendly and easy to navigate without the need for any technical knowledge. Features About RepairitĬurrently, Wondershare Repairit software is the number one video repair tool used by both young and old folks to fix corrupted video files conveniently. Repair video raw files to make them play smoothly without lagging or buffering.Repairs videos that a camera could not properly complete.Speedily recovers lost video data like header and metadata.Fix videos that don’t play on media players like QuickTime or Windows media player. ![]() Fix corrupted video files without tampering with their quality.Efficient repair tools do this by downloading the corrupt video file, scanning it, and retrieving the audio and visual aspects of the video that is not damaged.Īn efficient video repair tool can do the following Video repair tools are programs that can come in handy when the need to fix any kind of video-related problem arises. ![]() This is because such an event usually affects critical elements in the video files, thus, leaving them damaged. Videos are prone to get corrupted when they encounter an abrupt shutdown while being played. Playing or editing your videos using an incapacitated or malicious third-party video player/editor can badly damage your video files, as it might not have the required properties to manage/process the video files.
![]() Another is the side-by-side and split views to show your image before and after edits. One Affinity Photo interface feature I approve of is that double-clicking a control slider sets it back to its original state. You can zoom with the mouse wheel plus Ctrl, and double-clicking switches you back to Fit view. You can customize the workspace, and Serif has now added the ability to save custom workspaces, as you can in Adobe Photoshop. A simple workspace reset option under the main View or Window menu would be preferable. I finally discovered the option way down at the bottom of a long flyout list from the View > Studio menu. Personas equate to workspaces, but I had a hard time resetting the workspace to the default after removing some panels. Panorama is another persona, but it's only visible when you're stitching multiple images. The default personas are Photo, Liquefy, Develop, Tone Mapping, and Export. As with Photoshop, the interface shows a toolbar row across the left side of the program window and an info panel to the right for things like Layers, Histogram, Swatches, Adjustment, Transform-25 modules in all, any of which you can undock.Ībove the main image view, there are buttons for five Personas-what most programs call modes. The interface is industry-standard very dark gray, but its many colorful icons are less understated than most of the photo editing apps of today. Serif Photo's generous free trial gives you 90 days for the price of an email address that you confirm. There’s no upgrade pricing, but you can install the software on as many computers as you have Adobe limits Creative Cloud (such as Lightroom and Photoshop) applications to two machines. How Much Does Affinity Photo Cost?Īffinity Photo is available on the Mac App Store, from the Microsoft Store in Windows 10, and as a website download, for a one-time price of $49.99 (discounted to $24.99 at time of writing). Several of the program’s tools, too, fall short of Adobe's state-of-the-art software. It’s feature-packed and powerful, but some operations are still slow and the user interface still lacks some friendliness found in competitors. ![]() The latest version of this photo editing software adds hardware acceleration for improved performance on Windows, linked layers, astrophotography stacking, and custom interface presets. Serif’s low, non-subscription pricing is no doubt a factor for some users. The Affinity apps are also available for Windows, and the company is attempting to make inroads on Adobe's firm grip on the media and design software industry. Its Affinity line, which started with Mac-only apps in 2015, even garnered an Apple Mac App of the Year award that year. ![]() How to Set Up Two-Factor Authenticationīritish firm Serif has been in the media-creation-software space for over a decade.How to Record the Screen on Your Windows PC or Mac.How to Convert YouTube Videos to MP3 Files.How to Save Money on Your Cell Phone Bill.How to Free Up Space on Your iPhone or iPad.How to Block Robotexts and Spam Messages. ![]() ![]() General Safety Tips: By taking a few simple security precautions before and during your vacation you can help ensure that you, your traveling companions, and your valuables are as safe and secure as possible. You’ll find information on recent outbreaks, required vaccinations, insect protection, illness and injury abroad, and more. Travel Health Information: For the most current worldwide health information, consult your family physician or the official website for the Center for Disease Control at or by phone at 1-80. Packaged produce, such as pineapple, is available for purchase at the airport or in designated stores on the island. This brief procedure is designed to prevent travelers from exporting any uninspected local produce. For details, please consult your airline.ĭeparting: At the airport prior to check-in, travelers bound for mainland destinations will be required to pass their baggage through agriculture inspection. A 120-day quarantine is required for most pets and a number of plants are restricted. The importation of uninspected plants and animals could ruin Hawaii’s paradise forever. The agriculture information is necessary to maintain Hawaii’s delicate environment. This visitor information is used in tabulating visitor data. ![]() ![]() If baggage fees do apply, this cost will be additional and must be paid directly to the airline.Īrriving: Onboard your aircraft, all visitors will be given a form from the Hawaii State Department of Agriculture, and the Hawaii Visitors and Convention Bureau asking for information about their trip. For airline baggage fees and policies please visit. Luggage: Baggage allowances and fees vary by airline. Please bring this information with you if you are traveling on a honeymoon package. Honeymoon Packages: Some hotels require a marriage certificate or license for you to receive the honeymoon package. If not, you could be denied boarding and no refund will be forthcoming. Department of State at Newlywed Information: Tickets and other travel documents must exactly match your current identification. For the most current information, visit the website for the U.S. Rules are subject to change at any time, without notice. citizens traveling to or within the Hawaiian Islands. citizens must travel with a valid government-issued photo identification (such as a driver’s license). It is important to arrive at the airport at least 90 minutes prior to departure for all domestic flights, and two to three hours prior to departure for international flights. Please bring all your final documents with you. Find your Hawaii, find your paradise.Īirport Check-In: It is recommended that you reconfirm your flight information and check in for your flights at the airline’s website 24 hours prior to your scheduled departure. There is indeed an island for every personality. history at Pearl Harbor, stroll through the pineapple maze at the Dole Plantation, hike the Diamond Head crater for unobstructed views of Waikiki Beach, visit the new International Market Place, and watch big wave surfers tackle the Banzai Pipeline. Transforming the southern coastline is the city of Honolulu while monster waves rule the northern coast. The most populated in the chain, Oahu offers a unique blend of modern and ancient influences. Sandwiched between Kauai and Maui is the bustling island of Oahu. Witness an awe-inspiring sunrise at Haleakala, snorkel with sea turtles at Molokini, stroll the eclectic shops in Lahaina and witness a ceremonial cliff dive at Black Rock. Known for famous beaches and championship golf courses, Maui delivers an experience filled with magic and beauty to visitors. ![]() Maui is the second-largest island and just to the north of Hawaii Island. Hike Waimea Canyon and gaze at the Grand Canyon of the Pacific, trek the Koloa Heritage Trail for a glimpse of the past, visit local botanical gardens for their tropical treasures, tour cascading waterfalls, and helicopter out to the Na Pali coastline for the ultimate vantage point. With age comes beauty and the dramatic landscape of Kauai reflects this sentiment. Kauai is the oldest of the islands and keeps watch over the others from the northernmost point of the chain. Explore Hawaii Volcanoes National Park, tour a local coffee plantation, stroll seaside villages, travel back in time at Puukoholo Heiau National Historic Site, and stand at the southernmost point of the United States where nothing but ocean separates you from Antarctica. A temperamental youngest sibling of the family, this island is full of drama, adventure, and natural beauty. Hawaii Island is the largest of the islands and sits at the southernmost part of the chain. Discover the differences between these majestic islands and choose your paradise. ![]() ![]() Remember that the use of our images is subject to compliance with our Terms of Use. You can see that as your icons are stored there, Flaticon does not allow them for redistributed the adapted and modified icons which under CC-BY and any other open source licence because Flaticon acts like as if they were owners of any open source icons. Flaticon has its own licenses for the icons available on the site, and these allow you to use Flaticon content without having to credit the author, provided that you have purchased one of the available Premium subscription plans. In spite of it, as your icons are stored, they became sub-licenced and closed-source. Your icons are under MIT, but at the site of Flaticon, they are under CC-BY3: Your open source icons became closed-sourced under Flaticon License: You can see that Flaticon took the flags from you and acted like if they were owners of your icons. Vaginal contraceptive film - Free healthcare and medical icons Special Ungravity Gradient Merchandising License NEW Customize any physical or digital product with our icons. ![]() ![]() Flaticon acts like as if they were owners of your icons. I would like to know if you yourselves published or stored your icons in the Flaticon site?īecause your icons are under MIT, but at the Flaticon/Freepik site, although your icons be under CC-BYA3, they have been sub-licenced by Flaticon, and their licence forbids the users of modifying and redistributing the modified icons (including of Twemoji and of Google) for the open source icons theme, menacing them with copyright infringement process. ![]() ![]() ![]() That would create chaos when browsing, with information popping up that might be helpful to another user, but not to you. Multiple users meant many different cookies would be stored on a shared computer. ![]() Here’s how private browsing solved the problem. It was primarily designed to make it easier to use shared computers. For example, a web browser could sell user info to third-parties for marketing purposes.įor example, Google’s Incognito mode wasn’t created to mask or hide your identity. But sometimes you might not want that convenience, if storing that data might compromise your privacy. How? When a browser cache web-based content like usernames, passwords, and images, this helps to speeds up the login process. Web browsers can store data about users’ activities to make revisits easy. Hint: Think VPN or virtual private network. For instance, do you know that browsing history can still be accessed in most browsers when you browse in private mode? Chances are, you want privacy and safety when you go online. Open your browser and choose the ‘ …’ icon on the bottom right.įinally, please note that you may find slightly different approach from the above instruction depending on your browser version.It’s important to understand what a private browser does, and does not, do.To clear your cache on Internet Explorer (mobile/tablet) Make sure to only select the Temporary Internet files and website files box to avoid clearing important information. You can also access the menu by using the shortcut: Ctrl+Shift+Del. Make sure to uncheck Preserve Favourites website data and check Temporary Internet Files, then click Delete button at the bottom.Then, find Safety and select Delete Browsing History from the submenu.To clear the browser cache for Internet Explorer, get started by clicking the Gear icon in the top right to access the Tools menu.To clear your cache on Internet Explorer (desktop/laptop) Open Clear browsing data and ensure ‘ Cache’ is selected and choose Clear browsing data. ![]() Open your browser and choose the ‘…’ icon on the bottom right.To clear your cache on Microsoft Edge (mobile/tablet) Choose Clear and this will clear your cache.Choose Clear all history, or choose the elements you want to delete.Click the three dots icon in the top right of your browser window.To clear your cache on Microsoft Edge (desktop/laptop) From there, choose Clear History and Data and this will clear your cache.To clear your cache on Safari (mobile/tablet) Then, you can go to Develop → Empty Caches to clear Safari’s browser cache. To only empty Safari’s browser cache, you’ll need to enable the Develop menu by going to Preferences → Advanced and checking the Show Develop Menu in Menu Bar box.If you’re ok with clearing your entire browsing history (including cookies and visited pages), you can take the easy approach and just go to Safari → Clear History.To clear the browser cache for Safari, you have two options: ![]() To clear your cache on Safari (desktop/laptop)
![]() ![]() Cover the entire page with wide clear tape, front and back, and cut them out. Then photocopy yearbook pictures of each student and glue them above the names. Make student "photo tiles" for a seating chart-a great way to connect new student names with faces! Print student names in a small font, last name below first, with enough space for photos above the names. Photo Tile Seating Charts ( Marcia Krech, Jefferson City, MO) It is a great way to get to know them as they get to know the room. It becomes easy to see who cooperates with others, which students prefer to be loners, and how students approach this task. This provides an opportunity for her to observe students interacting with one another as they find answers to the questions. finding all the important areas of the room, taking notice of safety posters, finding safety equipment, finding make-up work, etc. Following the old Chinese saying of "I Hear, I forget I See and I remember I Do and I understand", the students "do" the room. Getting To Know Room 302 (submitted by Nancy Nega, Elmhurst, IL)ĭuring her "Getting To Know Room 302" activity, students work in pairs to scout out the room and find the answers to the "scavenger hunt". Day 1, Day 2, & Day 3 Student Survival Guide 2019 and check out the First Tips or free downloads areas. NOTE: I use several of the classroom procedures and an adapted version of the class rules from the Whole Brain Teaching method for classroom management. Tomm's Scavenger Hunt (pdf)įirst Day Presentation - Welcome to Mrs. This activity allows students and parents the chance to know my expectations from the start of the year. ![]() The assignment must be taken home and signed by a parent to earn a grade. After allowing the students time to gather the answers from signs and posters in the room, we discuss my expectations for the year as well as their questions and concerns. They also discover the location of the generic kleenex (toilet paper), extra supplies, reference books, etc. 8th Grade Scavenger Hunt ( Tracy (Trimpe) Tomm, Havana Junior High, Havana, IL)Īfter reviewing the class rules, my students complete a scavenger hunt to help them learn more about grading procedures, make-up, and computer rules. ![]() |
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